- Title
- Synthesis of poly(acrylamide)-based hydrogel for bio-sensing of hepatitis B core antigen
- Creator
- Lim, Swee Lu; Ooi, Chien-Wei; Low, Liang Ee; Tan, Wen Siang; Chan, Eng-Seng; Ho, Kok Lian; Tey, Beng Ti
- Relation
- Materials Chemistry and Physics Vol. 243, Issue 1 March 2020, no. 122578
- Publisher Link
- http://dx.doi.org/10.1016/j.matchemphys.2019.122578
- Publisher
- Elsevier BV
- Resource Type
- journal article
- Date
- 2020
- Description
- The major health issues posed by hepatitis B virus (HBV) globally have always been the impetus for the development of novel diagnostic assays. Here, we report the synthesis of bio-conjugated polyacrylamide-based hydrogel (HBPAAm hydrogel) that responds to the presence of hepatitis B core antigen (HBcAg) by a weight change in the hydrogel. The HBPAAm hydrogel contained the affinity crosslinks formed by the bio-specific coupling between the bound HBcAg and its antibody (anti-HBc). In the presence of analyte (i.e., free HBcAg), the immobilized HBcAg-(anti-HBc) complexes in HBPAAm hydrogel were disrupted; the competitive binding of free HBcAg onto the bound anti-HBc in HBPAAm hydrogel triggered the swelling of hydrogel. The swelling of HBPAAm hydrogel corresponded to the concentration of free HBcAg. The bio-sensing property of HBPAAm hydrogel is highly specific, and the reusability of HBPAAm hydrogel for multiple runs of HBcAg sensing was demonstrated. The bio-conjugated hydrogel has great potential as a sensing component of the biosensor that allows the detection of HBV serological markers at a lower cost and a shorter time.
- Subject
- hepatitis B core antigen; smart polymer; polymer hydrogel; diagnosis; biospecific; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1463355
- Identifier
- uon:46710
- Identifier
- ISSN:0254-0584
- Language
- eng
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